The major activities under each Objective




1.   Establishment of a medium capacity high precision Plant Phenomics Facility  and standardization of operation

i)       Procurement and installation of equipments, and development of automated medium throughput and high precision phenomics facility on turn-key basis at NRCPB, New Delhi to gain hands-on experience required for further expansion in number, capacity, serving facilities and crops

ii)     Establishment of high-volume and low-precision phenotyping facilities like rain-out shelters and environment-controlled green houses to evaluate germplasm and select the feeder material for high precision facility

iii)   Standardization of operations for growing rice plants under controlled environmental conditions, and imaging platforms for non-destructive precision phenotyping using known contrasts

iv)    Development of techniques for analysis of  fluorescence, bioluminescence, thermal and hyperspectral reflectance images, and development of multi-modal image processing techniques

v)     Correlation of morpho-physiological traits with multi-modal spectral images under varying levels of stresses to identify parameters for high-precision phenotyping of rice for  soil moisture deficit and low temperature stress tolerance

2.   Precision phenotyping of a contrasting set of rice germplasm for soil moisture deficit and low temperature stress tolerance and creation of phenome database

i)       Characterization of the available core germplasm for morpho-physiological traits in rain-out shelters and environment controlled green houses under varying stress levels and  identification of reference germplasm sets

ii)     Non-destructive precision phenotyping of  reference germplasm sets using the imaging platforms and creation of phenome database

iii)   Analysis of the spectrum of phenotypic variation in the chosen traits, establishing marker-trait association and adding value to the germplasm

iv)    Development of machine learning based pattern recognition techniques and predictive models for phenome expression

v)     Phenotyping of insertion mutants of rice and Arabidopsis to discover new genes and dissect pathways influencing  soil moisture deficit and low temperature stress tolerance phenome

3.   Identification of the role of candidate genes in  soil moisture deficit and low temperature stress tolerance through precision phenomics of transgenics

i)       Selection of candidate genes from the public database and from those identified in the NAIP project on  Bioprospecting of genes and allele mining for abiotic stress tolerance” and generation of constructs for their over-expression and down-regulation

ii)     Development of transgenic rice and Arabidopsis and their precision phenotyping  to determine the role of the candidate genes in stress tolerance

iii)   Identification of useful genes and transgenics for possible commercialization

4.   Human resource development in the area of Plant Phenomics

i)       Research level: Skill development of the project-associated scientist in the area of high throughput phenomics through international trainings

ii)     User/operational level: Imparting training to the National Stake Holders including project partners in the use of phenomic facility and non-destructive imaging technologies for germplasm evaluation, understanding stress tolerance and gene discovery